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APC Annexin V
APC Annexin V from BioLegend
(2)
Write a review
Citations:
(140)
Supplier Page
Supplier Page from
BioLegend for
APC Annexin V
Description
APC Annexin V
Product Specs
Company Info
Citations
Reviews
Videos
Product Specs
Item
APC Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640920
Quantity
100 tests
Applications
Flow Cytometry (FC)
Conjugate/Tag
APC
NCBI Full Gene Name
annexin A5
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (140)
(21)
Oncolytic Herpes Simplex Virus Type 1 Induces Immunogenic Cell Death Resulting in Maturation of BDCA-1(+) Myeloid Dendritic Cells
International journal of molecular sciences
April 27, 2022
Kalus P, De Munck J, Vanbellingen S, Carreer L, Laeremans T, Broos K, Dufait I, Schwarze JK, Van Riet I, Neyns B,
et al
.
were resuspended in 100 µL composed of 2 µL AnnexinV-APC (1/50;
BioLegend
, Amsterdam, Netherlands;
640920
), 3.3 µL
DAPI solution
(0.03 µg/mL) and 94.7 µL 1X Annexin V Binding Buffer (
BD Biosciences
, 55645).
(22)
Ca
2+
-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair
Nature communications
April 6, 2022
Niekamp P, Scharte F, Sokoya T, Vittadello L, Kim Y, Deng Y, Südhoff E, Hilderink A, Imlau M, Clarke CJ,
et al
.
µM ionomycin or 0.1% (v/v) DMSO for 10 min at 37 °C in 5% CO2. Next,
APC-Annexin V
(
Biolegend
, no.
640920
; 5 µl in 100 µl Binding Buffer) and propidium iodide (5 µg/ml;
Sigma-Aldrich
,
P4170
) were added and
(23)
Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liver
Science advances
March 18, 2022
Lee Y, DiMaulo-Milk E, Leslie J, Ding L.
(1 μg/ml). Annexin V
staining
was performed using Annexin V Apoptosis Detection Kit APC (
BioLegend
,
640920
) per the manufacturer’s instructions. Intracellular
staining
For intracellular
staining
, cells were
(24)
Integrated Multiomic Profiling Identifies the Epigenetic Regulator PRC2 as a Therapeutic Target to Counteract Leukemia Immune Escape and Relapse
Cancer discovery
March 7, 2022
Gambacorta V, Beretta S, Ciccimarra M, Zito L, Giannetti K, Andrisani A, Gnani D, Zanotti L, Oliveira G, Carrabba MG,
et al
.
V–positive cells at the end of the
assay
within the population of blasts (
BioLegend
,
APC Annexin V
; cat. #
640920
, lot #B334668). Untreated target cells served as a control to detect the basal proportion of apoptotic
(25)
Kupffer Cells and Blood Monocytes Orchestrate the Clearance of Iron-Carbohydrate Nanoparticles from Serum
International journal of molecular sciences
February 28, 2022
Arsiwala T, Vogt AS, Barton AE, Manolova V, Funk F, Flühmann B, Bachmann MF.
Franklin Lakes, NJ, USA), anti-
Ly6G
(#
17-9668-80
,
Thermo Fisher Scientific
, Waltham, MA, USA), annexin V (#
640920
,
BioLegend
San Diego, CA, USA), and Fixable viability dye (eFluorTM506 CYAN, #
65-0866-14
,
eBioscience
,
(26)
Specific overexpression of SIRT1 in mesenchymal stem cells rescues hematopoiesis niche in BMI1 knockout mice through promoting CXCL12 expression
International journal of biological sciences
January 1, 2022
Li J, Li X, Sun W, Zhang J, Yan Q, Wu J, Jin J, Lu R, Miao D.
analysis. To detect Annexin V,
CD44
and
CXCR4
expression on LSK surfaces, APC-conjugated Annexin V (#
640920
) or
CD44
(#
103012
) or
CXCR4
(#
146508
) from
BioLegend
were added in
staining
panel with LSK surface markers
(27)
A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics
Cellular and molecular gastroenterology and hepatology
January 1, 2022
Burclaff J, Bliton RJ, Breau KA, Ok MT, Gomez-Martinez I, Ranek JS, Bhatt AP, Purvis JE, Woosley JT, Magness ST.
RNAscope Multiplex Fluorescent Reagent Kit
v2,"
Advanced Cell
Diagnotics (Newark, CA)",323100 Antibodies,, AnnexinV-APC,
BioLegend
,
640920
Lysozyme,"Diagnostic Biosystems (Pleasanton, CA)",RP028 CFTR,
Cystic Fibrosis
Foundation,A570 Mucin
(28)
Microtubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells
Cell adhesion & migration
December 1, 2021
Roncato F, Regev O, Yadav SK, Alon R.
Annexin V Binding buffer
(
BioLegend
, Cat#
422201
), labeled with APC-conjugated Annexin V antibody (
BioLegend
, Cat#
640920
) for 15 min at RT, and analyzed using a
CytoFLEX flow cytometer
(Beckman Coulter). Analysis of effector
(29)
Red blood cells and their releasates compromise bone marrow-derived human mesenchymal stem/stromal cell survival in vitro
Stem Cell Research & Therapy
December 1, 2021
Ryan Christopher Dregalla, Jessica Ann Herrera, Edward Jeffery Donner
FITC (
Biolegend
, cat. #
328108
) to gate for MSCs, annexin-V-conjugated (AV) to APC (
Biolegend
, cat. #
640920
) to detect apoptotic cells and 7-aminoactinomycin D (7AAD) viability stain (
Invitrogen
, cat. #
00-6993-50
)
(30)
Modulation of FLT3-ITD Localization and Targeting of Distinct Downstream Signaling Pathways as Potential Strategies to Overcome FLT3-Inhibitor Resistance
Cells
November 3, 2021
Maximilian Fleischmann, Mike Fischer, Ulf Schnetzke, Colin Fortner, Joanna Kirkpatrick, Florian H. Heidel, Andreas Hochhaus, Sebastian Scholl
purchased from Promega, Madison, WI, USA.
For flow cytometry
, the anti-CD135 (#
313305
), anti-Annexin V (#
640920
), and human IgG1 PE isotype control (#
403504
) were purchased from
BioLegend
, San Diego, CA, USA. 2.4.
(31)
PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8
+
T cells at the expense of central memory
Cell reports
October 12, 2021
Cannons JL, Villarino AV, Kapnick SM, Preite S, Shih HY, Gomez-Rodriguez J, Kaul Z, Shibata H, Reilley JM, Huang B,
et al
.
,
Thermo Fisher
,Cat #
L34966
LIVE/DEAD™ Fixable Near-IR,
Thermo Fisher
,Cat #
L34976
Annexin V APC,
Biolegend
,Cat #
640920
Annexin V binding buffer
,
Biolegend
,Cat #
422201
CAL-101
,
Santa Cruz Biotechnology
,Cat #364453 "
CAL-101
,
(32)
Drug Repurposing to Identify a Synergistic High-Order Drug Combination to Treat Sunitinib-Resistant Renal Cell Carcinoma
Cancers (Basel)
August 6, 2021
Magdalena Rausch, Adriano Rutz, Pierre-Marie Allard, Céline Delucinge-Vivier, Mylène Docquier, Olivier Dormond, Paul J. Dyson, Jean-Luc Wolfender, Patrycja Nowak-Sliwinska
purchased from BD Bioscience (San Jose, CA, USA) or
Biolegend
(London, United Kingdom). Annexin V-APC (
640920
) and propidium iodide solution (
421301
) were used to analyze apoptosis and cell cycle distribution.
(33)
Interferon-α-producing plasmacytoid dendritic cells drive the loss of adipose tissue regulatory T cells during obesity
Cell metabolism
August 3, 2021
Li C, Wang G, Sivasami P, Ramirez RN, Zhang Y, Benoist C, Mathis D.
C10636
LIVE/DEAD™ Fixable Near-IR Dead
Cell Stain
Kit,
Invitrogen
,Cat#
L10119
Annexin V,
Biolegend
,Cat#
640920
Annexin V Binding Buffer
,
Biolegend
,Cat#
422201
SuperScript™ III Reverse Transcriptase
,
Invitrogen
,Cat#
(34)
TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling
Cell reports
July 13, 2021
Agarwal P, Li H, Choi K, Hueneman K, He J, Welner RS, Starczynowski DT, Bhatia R.
RRID:AB_10900447 Anti-mouse
CD182
(
CXCR2
),
BioLegend
,Cat#
149310
; RRID:AB_2566148 Anti-Annexin V,
BioLegend
,Cat#
640920
; RRID:AB_2561515 Anti-CD117 MicroBeads,
Miltenyi Biotech
,Cat#
130-091-224
; RRID:AB_2753213 "Ki-67 Monoclonal
(35)
Promoter usage regulating the surface density of CAR molecules may modulate the kinetics of CAR-T cells
in vivo
Molecular therapy. Methods & clinical development
June 11, 2021
Ho JY, Wang L, Liu Y, Ba M, Yang J, Zhang X, Chen D, Lu P, Li J.
streptavidin-APC-Cy7 (
405208
),
PD1
-FITC (
329904
),
TIM3
-PE (
345006
),
LAG-3
-APC (
369212
),
APC-Annexin V
(
640920
), and 7AAD (
420404
), which were purchased from
Biolegend
(London, UK). Antibiotin-PE was purchased from
(36)
Short lifespan of syngeneic transplanted MSC is a consequence of in vivo apoptosis and immune cell recruitment in mice
Cell death & disease
June 2, 2021
Preda MB, Neculachi CA, Fenyo IM, Vacaru AM, Publik MA, Simionescu M, Burlacu A.
estimate the effect of double labelling, 105 MSC were incubated with 5 µl
APC Annexin V
(
Biolegend
,
640920
) and 5 μg/ml
Propidium Iodide
(
Sigma-Aldrich
,
P4170
) in 100 μl, for 15 min in the dark. To determine
(37)
A Chemically Defined, Xeno- and Blood-Free Culture Medium Sustains Increased Production of Small Extracellular Vesicles From Mesenchymal Stem Cells
Frontiers in Bioengineering and Biotechnology
May 26, 2021
Aliosha I. Figueroa-Valdés, Catalina de la Fuente, Yessia Hidalgo, Ana María Vega-Letter, Rafael Tapia-Limonchi, Maroun Khoury, Francisca Alcayaga-Miranda
analysis. Then, cells were stained with Annexin V-APC (BioLegend, San Diego, CA, United States, Cat. #
640920
) and 7-aminoactinomycin D (7-AAD) (
BioLegend
, San Diego, CA, United States, Cat. #
420403
) in
Annexin V binding buffer
(38)
Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4
+
T cells
eLife
April 9, 2021
Cai J, Gao H, Zhao J, Hu S, Liang X, Yang Y, Dai Z, Hong Z, Deng K.
(39)
DNA hypomethylating agents increase activation and cytolytic activity of CD8
+
T cells
Molecular cell
April 1, 2021
Loo Yau H, Bell E, Ettayebi I, de Almeida FC, Boukhaled GM, Shen SY, Allard D, Morancho B, Marhon SA, Ishak CA,
et al
.
(40)
Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway
Nature communications
March 23, 2021
Fujino T, Goyama S, Sugiura Y, Inoue D, Asada S, Yamasaki S, Matsumoto A, Yamaguchi K, Isobe Y, Tsuchiya A,
et al
.
surface marker antibodies, as described previously, and were then stained with Annexin V-APC (
BioLegend
,
640920
) antibody in binding buffer (10 mM Hepes (pH 7.4), 140 mM NaCl, 2.5 mM CaCl2) for 30 min at room temperature. HSPC
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Reviews
Write a Review
(1)
8/6/2018
Annexin V APC for Determining Apoptosis in Cells
Suzanne Tomchuck
We often look at the effects of different compounds on cell viability and apoptosis. We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the...
Read Review
(1)
2/24/2017
Staining T Cells With Annexin V APC
Adam Guess
This reagent was used to characterize living versus apoptotic/necrotic cells. It was chosen because it is the gold standard for measuring apoptosis, APC is bright and works well with other ...
Read Review
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APC Annexin V from BioLegend
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